Fig. 5

Spatial analysis kidneys at 22 h and 27 days post-injection (pi). For each time point, 3 sections from 3 individual kidneys were included. Panels A-C show example segmentations of the tissues of interest on the phalloidin images. D) Uptake as a function of distance from outer tissue edge, relative to average activity concentration in the tissue section. At 27 days pi, a statistically significant accumulation in the cortex, but not the deeper medulla, is readily discernible. E) The blood pool activity concentration was significantly above tissue average at 22 h pi, but not at 27 days pi. F) The activity concentration around the glomeruli was significantly elevated at both 22 h and 27 days pi relative to the tissue average. At the later time point, the accumulation increased further, peaked at 75 micrometers, and was significantly elevated between ~ 5–200 micrometer distance from the glomeruli, relative to the earlier timepoint, indicating a mechanism of accumulation proximal (but not inside) the glomeruli. This effect cannot be explained by general cortical increase, or spill-over from the blood pool activity. Shaded areas indicate 95%-confidence interval.