Fig. 5

The results of the computer simulation of the cloning process are presented as follows: A:Computer-simulated cloning process;B:Map depicting the simulated cloning products; C:Results from the simulated agarose gel experiment (1.0% agarose), where the first lane represents the mRNA vaccine, the second lane corresponds to the plasmid pVAX1, and the third lane displays the cloned product. D:Map illustrating the amplification of the simulated mRNA vaccine;.