Fig. 2

Obesity modifies physical phenotypes and further regulates ferroptosis in skeletal muscle. (A) Representative DEXA-scanned images of NDC, HF, HFR, and HFRV mice; skeletal muscle, fat tissue, and bone are shown in blue, red, and white, respectively. (B) Weekly change in body weight (group × weeks): NDC vs. HF (*, 54–72 weeks). (C–H) Quantitative data of final total mass, lean mass, lean mass ratio, fat mass, fat mass ratio, and fat mass in tissue were acquired by DEXA scanning. (I–L) Skeletal muscle function tests. (I) Walking speed test. (J) Strength test. (K) Endurance test. (L) Physical activity test. (M) Skeletal muscle cross-sectional area in NDC and HF. (N) Skeletal muscle collagen area in NDC and HF. (O) 4-HNE expression levels in skeletal muscle. (P) MDA expression levels in skeletal muscle. (Q) Iron percentage in skeletal muscle, and (R): Representative images showing H&E staining, Masson’s trichrome staining, IHC, and Prussian blue staining of GAS. Scale bar = 50 μm. Significant differences are denoted by asterisks: P < 0.05 (*), P < 0.01 (**), P < 0.001 (***), and P < 0.0001 (****). All values are presented as mean ± SD. Normal diet control (NDC, n = 7; 20.5 months old), high-fat diet (HF, n = 7; 20.5 month sold).