Table 2 Primary and secondary antibodies.

From: Differential ferroptosis regulation in red and white gastrocnemius under obesity and its Attenuation by exercise and dietary restriction

Antibodies

Source

Vender

Catalogue

Dilution (WB)

Dilution (IHC)

P53

Rabbit

Cell Signaling

30,313

1:500

 

SLC7A11

Rabbit

Abcam

ab175186

1:1000

 

GAPDH

Rabbit

Cell Signaling

5174

1:4000

 

GPX4

Rabbit

Abcam

ab125066

1:2000

 

AMPK

Rabbit

Cell Signaling

5831

1:500

 

p-AMPK Thr172

Rabbit

Cell Signaling

2531

1:500

 

TFR1

Rabbit

Abcam

ab84036

1:500

 

NCOA4

Rabbit

Invitrogen

PA5-115626

1:500

 

Rab7

Rabbit

Abcam

EPR7589

1:1000

 

4-HNE

Rabbit

Abcam

ab46545

1:1000

1:200

MDA

Mouse

Abcam

ab243066

1:500

1:50

Goat anti-rabbit

Goat

Invitrogen

G-21,234

1:5000

1:500

m-IgG Fc

SANTA

sc-525,409

1:2000

1:25