Fig. 3
From: Contribution of reversible histone acetylation to freeze tolerance and recovery in wood frog kidneys
Relative protein levels of 9 acetylated histone residues in R. sylvatica kidneys as determined by Western immunoblotting. (A) Histogram shows mean (± SEM, n=4) standardized expression levels of H2AK5ac, H2BK5ac, H3K9ac, H3K14ac, H3K18ac, H3K23ac, H3K27ac, H3K56ac and H4K8ac under control, 24 h freezing and 8 h thawed conditions. (B) Representative western blots for individual histone marks under each experimental condition. Original blots are presented in Supplementary Figure S1. Data are mean ± SEM (n = 4 independent trials). Statistical significance for freezing and thawing values, relative to the standardized control, was determined using one-way analysis of variance (ANOVA) with Tukey’s post hoc test where a, b, c that share the same letters were not statistically different from one another (*—p < 0.05).
