Fig. 4
From: Contribution of reversible histone acetylation to freeze tolerance and recovery in wood frog kidneys
Relative protein levels of histone lysine deacetylases in R. sylvatica kidneys as determined by Western immunoblotting. (A) Histogram showing mean (± SEM, n=4) standardized expression levels of control, 24 h freezing and 8 h thawed conditions. For each HDAC, values that share the same letter designation are not significantly different from one another, while values with different letter designation are significantly different (p < 0.05). (B) Representative western blots for individual HDACs as well as p-HDAC4 and p-HDAC8 under each experimental condition. Data are mean ± SEM (n = 4 independent trials). Statistical significance for freezing and thawing values, relative to the standardized control, was determined using one-way ANOVA with Tukey’s post hoc test where a, b, c that share the same letters were not statistically different from one another (*—p < 0.05). Original full blots are presented in Supplementary Figure S1. .
