Fig. 7 | Scientific Reports

Fig. 7

From: Genome wide identification and characterization of EPFL genes and its potential association with male sterility in Brassica rapa

Fig. 7

Expression levels of BrEPFL genes in ogura sterile lines based on qRT-PCR. (A) and (B), Transverse sections of anther development in six periods (microspore mother cell, meiosis II, tetrad, uninucleate, binucleate, and mature pollen period) from B. rapa (Wucai) maintainer and ogu-CMS lines, respectively. AP, abortive pollen; AT, abnormal tapetum; DM, degenerated microspore; E, epidermis; En, endothecium; ML, middle layer; Mmc, microspore mother cell; MPG, mature pollen gains; Ms, meiocytes; Msp, microspores; T, tapetum; Tds, tetrads. Scale bar = 100 μm. C, qRT-PCR analysis of BrEPFL genes in meiosis II, tetrad, uninucleate, and binucleate period. Different lowercase letters above the bars indicate statistically significant differences among stages based on Duncan’s new multiple range test (p < 0.05).

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