Fig. 7
Nifedipine attenuated FSK-driven cyst formation. Cultured E12.5 kidneys were treated with 3 µM nifedipine and varying concentrations of FSK. Cyst formation was imaged and quantified after 2 days of culture. (a–f) Brightfield images of E12.5 kidney rudiments treated with 3 µM nifedipine and 3 µM nifedipine with 0.25 µM, 0.5 µM, 1 µM, 2 µM, 4 µM and 8 µM FSK. (g,h) Quantification of cystic areas and cyst numbers in E12.5 kidney rudiments after 2 days of 3 µM nifedipine with varying concentrations of FSK (green line) and FSK alone (blue line). Cysts were indicated by red arrows. In (g) and (h), data are means of at least 3 kidneys. Error bars indicate standard errors of the mean. p-values were calculated using unpaired t-tests. *p < 0.05, **p < 0.008, ns; not significant. The FSK data presented are reproduced from Fig. 1.
