Fig. 7
From: NOX4 serves as a pan-cancer prognostic biomarker and therapeutic target in tumorigenesis

Knockdown of NOX4 inhibits cell proliferation and migration in MDA-MB-231 cells. (A) Western blot analysis confirming NOX4 knockdown. (B) qRT-PCR validation of NOX4 mRNA suppression. (C) Cell proliferation assessed using the CCK-8 assay at 24, 48 and 72 h post-transfection. (D) Wound healing assay results at 0 and 24 h. (E) Transwell migration assay results after 48 h. Data are shown as mean ± SD from three independent experiments (n = 3 per group). Statistical significance was determined using unpaired Student’s t-test. p < 0.05 was considered significant.