Fig. 6
From: Mild hypothermia attenuates hepatic ischemia-reperfusion injury by regulating FoxO1/PPARα pathway
AS1842856 reduced FoxO1 and PPARα interaction. (A) Immunoprecipitation in BRL-3 A cells treated with AS1842856 followed by immunoblotting using anti-PPARα and anti-FoxO1 antibodies. (B) Representative immunofluorescence image of FoxO1 and PPARα in BRL-3 A cells treated with AS1842856 on H/R, scale bar = 10 μm. (C) Immunoprecipitation with anti-FoxO1 antibody in BRL-3 A cells followed by immunoblotting using anti-PPARα and anti-FoxO1 antibodies. (D) Representative immunofluorescence image of FoxO1 and PPARα in BRL-3 A cells treated with AS1842856 on MH + H/R, scale bar = 10 μm. (E) Immunoprecipitation with anti-FoxO1 antibody in BRL-3 A cells followed by immunoblotting using anti-PPARα and anti-FoxO1 antibodies.
