Table 1 Regression and validation parameters of the proposed HPLC method.

From: Development and validation of a RP-HPLC method for simultaneous determination of five COVID-19 antiviral drugs in pharmaceutical formulations

Parameters

Favipiravir

Molnupiravir

Nirmatrelvir

Remdesivir

Ritonavir

Wavelength (nm)

230

230

230

230

230

Linearity range (µg/ml)

10 ─ 50

10 ─ 50

10 ─ 50

10 ─ 50

10 ─ 50

- Slope (b)

- Intercept (a)

26.3796

− 8.6493

41.247

0.0259

13.929

− 1.576

29.116

− 14.512

16.647

− 0.748

Coefficient of determination(r2)

0.9997

0.9998

0.9999

0.9997

0.9999

LOD (µg/ml)

0.946

0.747

0.415

0.877

0.470

LOQ (µg/ml)

2.868

2.264

1.260

2.657

1.424

Trueness (%R)*

99.59 ± 0.82

99.87 ± 0.671

100.08 ± 0.54

100.05 ± 0.71

100.08 ± 0.47

Precision (%RSD)**

Repeatability

0.901

0.720

0.606

0.949

0.832

Intermediate precision

1.076

0.806

0.770

0.995

0.731

  1. *Average of nine determinations (triplicate determination of three concentration level).
  2. **%RSD of %Recovery of nine determinations (triplicate determination of three concentration level).
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