Fig. 9
Identification of transcription factor regulatory modules in cardiomyopathy smooth muscle cells (A) The heatmap displayed the identification of regulatory submodules in smooth muscle cell subpopulations associated with cardiomyopathy based on SCENIC regulatory rules modules and AUCell scores similarity. Eventually, two rule-based submodules were identified. (B, C) UMAP plots and faceted plots showed the distribution of AUC scores of two rule-based submodules (M1, M2) and their distribution across various smooth muscle cell subpopulations (left). Box plots intuitively compared the expression levels of M1 and M2 in each smooth muscle cell subpopulation (middle). Scatter plots provided a better understanding of the expression score rankings of M1 and M2 in each smooth muscle cell subpopulation (right). (D) Scatter plot displayed the ranking of different TFs in M1 based on their variation scores. (E) Visualization of the 5 TFs with high variation scores in M1. UMAP plots illustrated the expression distribution of JUNB, ATF3, FOS, FOSB, and JUN, while box plots compared their expression differences among the nine smooth muscle cell subpopulations. (F) Scatter plot depicted the variation scores of different TFs in M2. (G) Visualization of the 2 TFs with high ranking in M2. UMAP plots respectively showed the expression distribution of these TFs, while box plots compared their expression differences among the nine smooth muscle cell subpopulations. (H) Scatter plots showed the ranking of the main cell cycle phases in M1 and M2. (I) Scatter plots showed the ranking of the groups to which cells belonged in M1 and M2.
