Table 1 Immunoassays used for analysis of NP cleavage.
From: Neprilysin 2 catalyses the degradation of natriuretic peptides despite sacubitrilat Inhibition
Immunoassay name | Antibody specificities | Schematic representation of the immune complex |
|---|---|---|
Intact-ring ANP s-IA | Antigen: ANP with intact ring structure Capture: mAb 23/1 (ANP aar 8–15) Detection: pAb to ANP ring structure (aar ANP 15–23) |
|
Intact-ring BNP s-IA | Antigen: BNP and proBNP with ring structure intact within aar 14–21 of BNP (90–97 of proBNP) Capture: mAb 50E1 (BNP aar 26–32, proBNP aar 102–108) Detection: mAb BNP130 (BNP aar 14–21, proBNP aar 90–97) |
|
Neo5 s-IA | Antigen: BNP with the neo-epitope BNP neo5 Capture: pAb Neo5 (BNP neo5 epitope) Detection: mAb 50E1 (BNP aar 26–32, proBNP aar 102–108) |
|
Neo17 s-IA | Antigen: BNP or proBNP with a cleaved ring structure between R17 and I18 Capture: MAb BNP503 (BNP neo17 epitope) Detection: mAb 50E1 (BNP aar 26–32, proBNP aar 102–108) |
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