Fig. 3 | Scientific Reports

Fig. 3

From: The inner nuclear membrane protein, Banf1, has an essential role in triple negative breast cancer cell proliferation and survival

Fig. 3The alternative text for this image may have been generated using AI.

Banf1 induces aberrant NE morphology in TNBC cells. Representative immunofluorescent microscopy images of MCF10A and TNBC cells transfected with Control and Banf1 siRNAs. Cells were stained with anti-Lamin A/C to visualise the NE. Cells were counterstained with Hoechst 3342 (blue). (a) MCF10A (c) BT549 (e) Hs578T (g) MDA-MB-231 and (i) MDA-MB-468. Quantification of cells with aberrant nuclear morphology in Control and Banf1 transfected cells. Form Factor score of 1 = perfect round nucleus. Quantifications are based on 200 cells/condition per repeat. Error bars denote S.D. Normalised nuclear form factor values for: (b) MCF10A (d) BT549 (f) Hs578T (h) MDA-MB-231 and (j) MDA-MB-468. (k - l) Representative images of immunofluorescence staining of α-tubulin (green) in representative TNBC cell lines 72 h post-transfection with control and Banf1 siRNA. Cells were counterstained with Hoechst 3342 (blue) nuclear staining. (k) MCF10A (l) BT549. Statistical significance was calculated using unpaired t-test: ***, < p 0.0002, *, p < 0.0332 (n=3 for all experiments).

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