Fig. 4

siRNA mediated Banf1 depletion inhibits TNBC cellular proliferation and induces cell death. (a-e). Representative proliferation curves from 72 h post transfection with Control and Banf1 siRNAs using the Incucyte S3 live cell imaging system in MCF10A and TNBC cells. (a) MCF10A (b) BT549 (c) Hs578T (d) MDA-MB231 and (e) MDA-MB-468 (f – j) Relative area under the proliferation curve for (a – e) from independent experiments. (k-o) Graphs represent percent of viable cells post transfection with Control, Banf1 #1 and Banf1 #2 siRNA (k) MCF10A (l) BT549 (m) Hs578T (n) MDA-MB-231 and (o) MDA-MB-468. Values are normalised back to Control siRNA for respective cell lines. All graphed values represent results from individual repeats and error bars denote S.D. Statistical significance was calculated using unpaired t-test: ****, < p 0.0001, ***, < p 0.0002, **, p < 0.0021, *, p < 0.0332 (n=3 for all experiments).