Fig. 5

Evaluation of the ability of mucosomes to disrupt established biofilms. (a, e) Dose-dependent quantitative effect of each treatment on P. aeruginosa (a) and S. aureus (e) biofilms. (b, f) SEM on P. aeruginosa (b) and S. aureus (f) biofilms. Images acquired at 6k× magnification (scale bars = 2 μm), insets at 15k× (scale bar = 1 μm). (c, g) CLSM 3D projections of P. aeruginosa (c) and S. aureus (g) biofilms. The images acquired at 63× magnification (scale bars = 50 μm) highlight the biofilm matrix (stained in blue), viable bacteria (stained in green), and dead bacteria (stained in red). (d, h) Quantification of the blue-channel fluorescence intensity for P. aeruginosa (d) and S. aureus (h) biofilms from CLSM acquisitions.