Fig. 2
Meandro’s pipeline enables analysis of electroporated cells and their progeny in regenerating limbs. (A) Top: Algorithm scheme for image analysis. Bottom: Detail of the upper image. At each position along the proximal–distal (PD) axis, the local tangent is calculated based on nearby points along the axis. Subsequently, the orthogonal line is computed, and the number of pixels that have an intensity above the threshold. This information is then assigned to the corresponding position along the axis. The outcome is a one-dimensional representation of the signal in the limb. (B) Software pipeline: (i–iii) the Region Of Interest (ROI) is detected by AI and fine-tuned by the user. (iv) the user sets the PD axis manually. (v–vii) the software reads fluorescent signals above a user-defined threshold. (viii) cell density along the PD axis plot. AP Amputation plane.
