Fig. 3

Overexpression of Tig1 and Prod1 broadens spatial cell density distributions and shifts them proximally. (A–C) Spatial distributions of labelled cells along the proximal–distal (PD) axis of the regenerating limb of the axolotl at different times post electroporation for the Control (n = 3), Tig1 (n = 4), and Prod1 (n = 4) conditions. The continuous green line and the green shaded area represent the mean and the standard deviation, respectively. The coloured continuous line represents the fit of the distributions to a Gaussian for each condition: Control (black), Tig1 (orange) and Prod1 (blue). The fits were performed by calculating the first two moments of the experimental distribution. (D) Difference between the means of the distributions at each time point, with the color code consistent with (A–C). (E) Standard deviation versus time for control (black), Tig1 (orange) and Prod1 (blue).