Fig. 3 | Scientific Reports

Fig. 3

From: Establishment and rescue of fibroblast cell lines carrying a nonsense mutation of RB1 by CRISPR-based base editing

Fig. 3The alt text for this image may have been generated using AI.

RB1 gene disruption changes cell behavior and regulation. (A) Expression level of cell cycle regulation-engaged genes in WT and R552X cells measured by qPCR from nine independent experiments (n = 9; mean ± SEM). The average expression level of WT cells was normalized to 1, and the expression levels of He- and Ho-R552X were adjusted accordingly. (B) Relative cell proliferation rate. Cell expansion rate of each cell line was calculated using 15 independent measurements (n = 15; mean ± SEM) up to day 3. (C,D) Measurement of cell cycle progression changes in RB1-disrupted cell lines. Each cell line was stained with propidium iodide and each phase in the cell cycle were analyzed proportionally using flow cytometry across four independent experiments (n = 4; mean ± SEM). P values were obtained using one-way analysis of variance (ANOVA) and Tukey’s test for post hoc analysis: *P < 0.05, **P < 0.01, ***P < 0.001.

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