Fig. 3 | Scientific Reports

Fig. 3

From: Lycium barbarum glycopeptide mitigates retinal ischemia-reperfusion injury through its anti-inflammatory, anti- senescence, and anti-apoptosis properties

Fig. 3The alternative text for this image may have been generated using AI.

Anti-senescence effects of LbGp. (A) SA-β-gal showing the state of optic nerve senescence in each group of mice. Red and blue coloring for healthy and aged tissues, respectively. (B) IF demonstrates LbGp-mediated modulation of p21 expression in the retinas of RIRI mice, scale = 50 μm. Dual fluorescence labeling reveals p21 (red) and nuclear DNA (blue) distribution patterns in retinal tissues. (C) IF demonstrates LbGp-mediated modulation of p16 expression in the retinas of RIRI mice, scale = 50 μm. Dual fluorescence labeling reveals p16 (red) and nuclear DNA (blue) distribution patterns in retinal tissues. (D) Quantitative analysis of p21 and p16 fluorescence intensity in mice. Values represent mean ± SD from triplicate experiments: **p < 0.01 vs. Sham group, #p < 0.05 vs. RIRI group. (E) IF demonstrates LbGp-mediated modulation of p21 and p16 levels in OGD/R cells, scale = 20 μm. Triple fluorescent labeling reveals the distribution pattern of p21 (red), p16 (green), and nuclear DNA (blue) in cells. (F) Quantitative analysis of cellular p21 and p16 fluorescence intensity. Values represent mean ± SD from triplicate experiments: **p < 0.01 vs. Ctrl group, ##p < 0.01 vs. OGD/R group.

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