Fig. 7
From: Hyperglycemia-induced mitochondrial abnormalities in autonomic neurons via the RAGE axis
Detection of RAGE protein in mitochondria-enriched samples from PC12NGF cells. A-B, Immunoblots and quantification of the level of expression of the neuronal markers MAP-2, neurofilament light chain (NF-L) and tubulin in whole PC12 samples maintained in media without (-NGF) or with NGF added (+ NGF). C, The immunoblots show the levels of RAGE, VDAC and tubulin detected in the cytosol-enriched and mitochondria-enriched samples from PC12 cells transformed by NGF (PC12NGF) maintained in either control (CTL) or high glucose (HG) conditions. C-D, The bar graphs show mean ± SEM levels of each protein after normalization to tubulin or VDAC in cytosol-enriched and mitochondria-enriched samples, respectively. Means were statistically compared by the Mann-Whitney U test; (MAP2: t4 = 2.695; NF-L: t4 = 3.297); * p < 0.05. (N = 3 per group in A and C). For clarity, the blots shown in A and C have been cropped, and the background brightness has been adjusted (complete blots available in Supplementary Data file).
