Table 1 PCR amplification of the two primer pairs, MatK and RbcL regions23.

From: Conservation of Verbascum sinaiticum Benth using innovative tissue culture technique and DNA barcoding

Loci

Primer

Sequence (5’->3’)

Product size(bp)

Primer concentration (pmol/uL)

Reference

matK

matK−390 F

CGATCTATTCATTCAATATTTC

600 bp Over

10

24

matK−1326R

TCTAGCACACGAAAGTCGAAGT

rbcL

rbcL−1 F

ATGTCACCACAAACAGAAAC

600 bp Over

10

14,25

rbcL−724R

TCGCATGTACCTGCAGTAGC

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