Table 1 Summary of the determined spectral markers and their corresponding parameters (band positions in the descriptors and integration ranges in cm−1).

From: Marker-independent vibrational spectroscopy imaging recognizes the hypoxia effect in the human brain endothelium

 

Assignment to biomolecules

Descriptor of the marker

Band integration range

 

Lipids

FTIR

Total lipids

(I2850 + I2922)/I2960

I2850: 2833–2870; I2922: 2903–2945; I2960: 2940–2985

Cholesterol

I1058/I2960

I1058: 1046–1071; I2960: as above

Esterified lipids

I1740/I2960

I1740: 1726–1756; I2960: as above

Phosphorylated lipids

I1232/I2960

I1232: 1213–1258; I2960: as above

Acyl chain length

I2850/I2960

I2850: as above ; I2960: as above

Proteins

Total proteins

(I1650 + I1540)/I2960

I1650: 1612–1668; I1540: 1524–1565; I2960: as above

Conformational changes

I1650/I1540

I1650: as above; I1540: as above

Serine (Ser) and threonine (Thr)

I1172/I2960

I1172: 1132–1190; I2960: as above

Tyrosine (Tyr)

I1513/I2960

I1513: 1480–1525; I2960: as above

Nucleic acids

DNA

I962/I2960

I962: 947–977; I2960: as above

RNA

I994/I2960

I994: 981–1014; I2960: as above

DNA/RNA

I962/I994

I962: as above; I994: as above

Raman

Lipids

Unsaturation of lipid droplets

I1660/I1440

I1660: 1608–1714; I1440: 1400–1505

Lipids

I2850/I1440

I2850: 2820–2863; I1440: as above

Proteins

Protein cross-linking

I1040/I1005

I1040: 1025–1065; I1005: 986–1030

Nucleic acids

DNA

I790/I1440

I790: 770–810; I1440: as above

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