Fig. 1
Validation of the conditional and constitutive Tmprss9 KO mice. (a) Scheme of the Tmprss9 wildtype (WT) and knockout (KO) alleles. (b) Genotyping of wildtype (WT, 788 bp and 498 bp), heterozygous mutant (+/-, 788 bp, 498 bp and 230 bp) and homozygous mutant (-/-, 230 bp) mice in the presence (WT, heterozygote) or absence of exon 3-containing PCR-amplified fragments. (c) Spleen mRNA transcript expression of Tmprss9 in WT (+/+, filled circles, n = 6–10), floxed (lox/lox, triangles, n = 5) and KO (-/-, open squares, n = 7–11). (d, e) mRNA transcript expression of Tmprss9 in kidneys from WT and KO on standard (SD), low K+ (LKD) or high K+ (HKD); blue columns, male; red columns, female mice. Values are mean ± SD. P values < 0.05 were considered statistically significant using one-way ANOVA with Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, difference between genotypes; # P < 0.05, ### P < 0.001, difference between diet conditions.
