Fig. 3

Slc12a1 mRNA transcript expression was greater in female Tmprss9 KO mice on standard diet. (a, c) Relative mRNA transcript expression of Romk1, (b, d) Romk2, (e, g) Slc12a1, (f, h) Slc12a3, (i, k) Scnn1a and (j, l) Scnn1g in kidneys from (a, b, e, f, i, j) male (blue) and (c, d, g, h, k, l) female (red columns) WT (filled circles) and KO (open squares) mice on SD, LKD and HKD. Values are mean ± SD (n = 5–7). P values < 0.05 were considered statistically significant using one-way ANOVA with Tukey’s multiple comparison test: * P < 0.05, difference between genotypes; # P < 0.05, ## P < 0.01, ### P < 0.001, difference between diet conditions.