Fig. 4

Phosphorylation of NCC was selectively increased in male and female Tmprss9 KO mice on low K⁺ diet but similar to WT mice. (a, b) Western blot analysis of NCC, p53-NCC and p58-NCC in kidneys from (a) male and (b) female WT and KO mice and, (c-l) their quantification of male (c, e, f, i, j, blue) and female (d, g, h, k, l, red column) WT (filled circle) and KO (open square) mice on LKD. β-actin was used as loading control. Values are mean ± SD (n = 5–7, each group). P values < 0.05 were considered statistically significant using one-way ANOVA with Tukey’s multiple comparison test; ^# P < 0.05, ^## P < 0.01, ^### P < 0.001, difference between diet conditions. Uncropped Western blots are presented as Supplementary information.