Fig. 5
NCC abundance and T58-phosphorylation were similar in WT and KO mice on SD and HKD. (a, b) Western blot analysis of NCC, p53-NCC and p58-NCCin kidney of (a) male and (b) female WT and KO and, (c-l) their quantification of male (c, e, f, i, j, blue) and female (d, g, h, k, l, red column) WT (filled circle) and KO (open square) mice on SD and HKD. β-actin was used as loading control. Values are mean ± SD (n = 6, each group). P values < 0.05 were considered statistically significant using one-way ANOVA with Tukey’s multiple comparison test; # P < 0.05, ## P < 0.01, ### P < 0.001, difference between diet conditions. Uncropped Western blots are presented as Supplementary information.
