Table 1 List of plasmid combinations used for the BiFC assay.

From: Identification of host cell surface proteins inhibiting furin dependent proteolytic processing of viral glycoproteins

Combination number

Plasmid 1

Plasmid 2

Plasmid 3

1

pTwist-EF1α-nCoV2019-S-2xStrep

pCAGGS-YN-GST

 

2

pLVX.IRES-Puro

pCAGGS-YC-GST

 

3

CD509B-TMPRSS2

pCAGGS-YC-GST

 

4

CD509B-TMPRSS2

pCAGGS-YC-GST

pLVX-IRES-Puro-PROM1

5

CD509B-TMPRSS2

pCAGGS-YC-GST

pLVX-IRES-Puro-AXL

6

pTwist-EF1α-nCoV2019-S-2xStrep

pCAGGS-YN-GST

pLVX-IRES-Puro-PROM1

7

pTwist-EF1α-nCoV2019-S-2xStrep

pCAGGS-YN-GST

pLVX-IRES-Puro-AXL