Fig. 4

Saturation of germination with red diode laser irradiation. Wild-type Arabidopsis seeds were irradiated with a cw red diode laser (DLR, 660 nm, 6000 µmol m– 2s– 1) for longer (A, 10–1000 s) or shorter (B, 0.1–10 s) irradiation time periods and germinated for 72 h in the dark at 22 °C or kept under constant white light (FWL). The percentage of germination was determined in three replicates with the total number of seeds per treatment group N > 300 and N > 65 per replicates in a subgroup (mean ± SE; n = 3). The line bisecting each box represents the median value; the lower and upper edge of the box indicate the minimum and the maximum values, respectively. The empty square within each box indicate the mean value. The letters a–d indicate significant differences in the means (p < 0.05 by ANOVA followed by a Tukey Test).