Fig. 4
From: Liquid biopsy of embryo culture medium for assessing human embryo viability using Raman spectroscopy

(a) Raman spectra in the 1500–600 cm−1 region for control culture medium A with pH values ranging from 5 to 9. (b) Plot of PC 1 scores vs. pH and (c) loading plot of PC 1 from PCA. (d) Titration curve of the culture medium with 1.0 mol/L HCl or NaOH. Positive and negative x-axis values represent the volumes of NaOH and HCl added, respectively.