Fig. 3

Cholesterol utilization and Bile salt hydrolase activity test. (A) In-vitro cholesterol utilization by the RSE02 strain in different time (0, 6, 12, 18, 24 and 48 h) interval, where initial cholesterol concentration was 100 µg/mL in minimal medium. (B) Bile salt hydrolase (BSH) activity of the RSE02 strain was assessed by the qualitative direct plate assay.