Fig. 5 | Scientific Reports

Fig. 5

From: Interfering with USP50 expression inhibits macrophage pyroptosis in sepsis-induced acute lung injury by degrading NLRP3 protein

Fig. 5

Silencing of USP50 attenuates lung injury in CLP mice via inhibiting pyroptosis. (A) USP50 expression in the lungs of mice in each group was measured by qPCR. (B) Lung injury was evaluated using H&E staining assay. (C) The wet/dry ratio of lungs was quantified. (D) IL-1β and (E) IL-18 levels in the serum of mice were measured using ELISA. (F) ASC, NLRP3, GSDMD, GSDMD-N, pro-caspase-1 and cleaved caspase-1 protein levels in the lungs of mice were examined using immunoblotting. (n = 6). Original blots are presented in Supplementary Fig. 4. **P < 0.01.

Back to article page