Fig. 6
Metabolomics Mechanism Map and Joint Multi-Omics Analysis. (A) Nitric oxide-citrulline cycle mechanism. Red represents DEMs. Arginine succinate is converted to L-arginine, generating fumarate. L-arginine produces NO via NOS. Electrons within NOS are generated through the oxidation of NADPH and transferred to haemoglobin, promoting NO production from L-arginine through the regulation of FAD and FMN. L-glutamate regulates the opening of cytosolic Ca2⁺ receptors, encouraging more Ca2⁺ to enter the cell and bind with CaM, thereby increasing NOS activity. This figure was drawn in the Generic Diagramming Platform. (B) Correlation diagram of DEGs and DEMs, where red denotes a positive correlation and blue signifies a negative correlation. A deeper hue indicates a stronger relationship. (C) Differential Gene-Differential Metabolite-Pathway Network Diagram. Orange dots represent differential genes, blue dots represent DEMs in positive ion mode, green dots represent DEMs in NEG, and the larger the dot, the higher the node degree. Pink arrows indicate pathways constituting the network with differential genes, while pink diamonds represent pathways constituting the network with differential metabolites. The hexagonal shape represents the 'Arginine and proline metabolism’ pathway, which is jointly enriched by differential genes and metabolites.
