Fig. 4
Relative gene expression of Collagen Type III Alpha 1 Chain (COL3A1) and Collagen Type I Alpha 1 Chain (COL1A1), Collagen staining, and Fibroblasts count evaluated in 3D EpiDerm FT models treated for 24 h with S-HFC (a, b, e, g) and F-HFC (c, d, f, h) respectively, at different concentrations (47.00; 28.20; 23.50 µgHyp/mL, corresponding to 0.1%; 0.06% and 0.05% w/v of HFC), with respect to controls (CTRL for S-HFC, consisting in vehicle, and 0.00 µgHyp/mL for F-HFC consisting in the cream without addition of HFC). In the box-and-whisker plots the horizontal line indicates the median, the box reflects values from the first to the third quartile, and the whiskers represent the furthest points that are not outliers. Values come from at least 2 independent experiments performed in triplicate. The significant differences are indicated by symbols on bars (**** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, * p ≤ 0.05 vs. CTRL).
