Fig. 2
From: Effects of SLC6A8 mutation-induced creatine deficiency on cellular function in fibroblasts
Subcellular proteomic analysis of CRT-G561R fibroblasts. (A–C) Volcano plots representing proteins in the cytosol (A), crude membrane (B), and plasma membrane (C) fractions of CRT-G561R (G561R) fibroblasts that were differentially expressed compared to those in CRT-WT (WT) fibroblasts. Protein abundance was quantified using SWATH–MS-based quantitative proteomics (n = 3 independent biological samples). p-values from t-tests were plotted against the fold change (G561R/WT) in protein expression. The horizontal dashed line represents the significance threshold (p = 0.05). The vertical dashed lines indicate the fold-change thresholds (0.5 and 2.0). Data were obtained from three independent biological replicates. The numbers of differentially expressed proteins (DEPs) (p < 0.05; fold change < 0.5 or > 2.0) are indicated. (D) Venn diagram illustrating the overlap of differentially expressed proteins across the cytosolic, crude membrane, and plasma membrane fractions of CRT-G561R fibroblasts. Total: number of differentially expressed proteins. Cyto: cytosol fraction, CM: crude membrane fraction, and PM: plasma membrane fraction.
