Fig. 3

Identification, screening, lentiviral packaging, and transfection of overexpressing and interfering periostin plasmid vectors. (A) Periostin expression vector map. (B) Periostin interference vector map. (C) The expression of periostin mRNA tested by QPCR 72 h after transfection. (D) The expression of periostin protein tested by Western Blot 72 h after transfection. (E) The result of the 1% agarose gel electropporesis. (F) The expression of periostin protein and mRNA tested by Western Blot and QPCR after lentiviral infection and puromycin screening.