Fig. 5
From: A digital plating platform for robust and versatile microbial detection and analysis
Enrichment and identification of bacteria in complex samples by coupling the DP with selective and differential medium. (A) Enrichment of S. aureus from a microbial mixture consisting of S. aureus and GFP-expressing E. coli by coupling the DP platform with a NaCl-based selective medium. (i) Representative bright-field, fluorescence, and merged microimages of microcolonies formed in two PicoArray devices after being covered with LB medium agar sheet and selective medium agar sheet (incubated at 37 ºC for 8 h), respectively. Initial concentrations of S. aureus and E. coli ~ 3 × 105 CFU/mL and ~ 5 × 104 CFU/mL (ii) Enumeration of the fluorescent and non-fluorescent microcolonies formed in the PicoArray devices with LB medium agar sheet and selective medium agar sheet, respectively. (B) Representative bright-field microimages of microcolonies formed in three PicoArray devices after being loaded with S. aureus, E. coli, and S. aureus/E. coli mixture and incubated with MCA sheets at 37 ºC for 8 h.
