Fig. 8 | Scientific Reports

Fig. 8

From: RNA-seq analysis of wild-type and mutated TBPL1 gene in breast cancer cells lines through CRISPR/Cas9 approach reveals novel molecular signatures

Fig. 8

Gene expression profiles of the wild type and TBPL1 knocked out gene determined in healthy and breast cancer cell lines. Knock-out cell lines were obtained by transfection of the U6-gRNA Cas-92A + GFP-RM CRISPR plasmid with Lipofectamine as described in Materials and Methods. Comparisons of RNAseq data were then carried out between non-transfected and transfected cell lines. Volcano plot of the genes identified in MCF-12F (A), T47D (B), SK-BR-3 (C), and MDA-MB-231 (D). (E) Principal Component Analysis (PCA) of the data obtained for the three replicates for each cell line. (F) Hierarchical clustering of the genes found overexpressed and subexpressed in the healthy and breast cancer cell lines.

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