Fig. 2 | Scientific Reports

Fig. 2

From: Proteomic analysis of tumor cell nuclear expulsion reveals significant cell adhesion and RNA binding programs in extracellular chromatin

Fig. 2

Profiling of citrullinated proteins in lung and breast cancer TuNEPs. (a) list of citrullinated peptides in TuNEPs compared with ApoDBs from PC9 cells with calcium- vs. without calcium-PBS respectively. (b and c) volcano plot (b) and list (c) of differential citrullinated peptides in TuNEPs compared with ApoDBs from MDA-MB-231-LM3 vs. MDA-MB-231 parental cells respectively. 10 μm Raptinal treated. For volcano plot, AbsLogFC > 0.5, p < 0.05, 369 citrullinated peptides with 1 peptide, 1%FDR. For list, changes are Log2FC > 0.6 and p value < 0.05), Xcorr > 1.7, and with unambiguous localization. Citrullinated proteins in high confidence were called when site localization scores > 90, no N or Q adjacent flanking R, or with medium confidence with site localization scores > 90, N or Q adjacent flanking R.

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