Fig. 3 | Scientific Reports

Fig. 3

From: Proteomic analysis of tumor cell nuclear expulsion reveals significant cell adhesion and RNA binding programs in extracellular chromatin

Fig. 3

Profiling of TuNEP-abundant proteins and citrullination compared to NETs. (a and b) volcano plot (a) and GO pathway analysis (b) of differential proteins from MDA-MB-231 LM3 derived TuNEPs compared with neutrophil derived NETs. (c–e) volcano plot (c), GO pathway analysis (d), and list (e) of differential citrullinated peptides from MDA-MB-231 LM3 derived TuNEPs compared with neutrophil derived NETs. 5 μm A23187 ionophore treated. For volcano plot, AbsLogFC > 0.5, p < 0.05, 2430 of whole proteins and 361 of citrullinated peptides with 1 peptide, 1%FDR. For list, changes are Log2FC > 0.6 and p value < 0.05), Xcorr > 1.7, and with unambiguous localization. Citrullinated proteins in high confidence were called when site localization scores > 90, no N or Q adjacent flanking R, or with medium confidence with site localization scores > 90, N or Q adjacent flanking R.

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