Table 1 Establishment of experiments.

Mold design and Multiphysics COMSOL

modeling experiment

Used Google SketchUp software to design a milli-fluidic device and simulate in Multiphysics COMSOL simulation software

To create a digital model of a milli- fluidic device and check the flow rate and the pressure of the system

PDMS fabrication

Use PDMS to fabricate a milli-fluidic device based on the design created in the previous step

To physically create a milli-fluidic device that can control the flow rate of the chemical Alginate with carbon nanotube

Development of Inf union pump

Use an extrusion pump to pump the chemical alginate with carbon nanotube into a calcium chloride jar through the milli-fluidic device

To generate microfibers using the chemical alginate with carbon nanotube by controlling the flow rate through the milli-fluidic device

Fourier Transform Infrared Spectroscopy FTIR

Used FTIR to analyze the chemical composition of the microfibers generated.

To identify the chemical composition of the microfibers generated and to understand the chemical interactions between the alginate and carbon nanotube.

Raman spectroscopy

Used Raman spectroscopy to analyze the chemical composition of microfibers generated

To understand the chemical interactions between the alginate and carbon nanotube.

X-ray diffraction analysis

Used X-ray diffraction machine to analyze the crystal structure of microfibers generated

To understand the crystal structure of the microfibers generated and to understand how the structure affects the properties of the microfibers.

I-V measurement

Used to measure Current and Voltage of the microfibers

To understand their electrical properties, particularly conductivity, which is essential for applications in nerve tissue engineering where electrical signal transmission is crucial

Nerve regulation application

Used to apply microfibers generated.

To explore their potential in supporting or enhancing nerve tissue regeneration and function. These microfibers provide a conducive environment for nerve growth and repair, and their conductive properties, enhanced by CNTs, could aid in transmitting electrical signals, which are essential for nerve function.