Fig. 6
From: Efficient cytoplasmic cell quantification using a semi-automated FIJI-based tool
(a) Representative images of mouse islets of Langerhans, exhibiting high background staining. Nuclei are stained with DAPI (blue), insulin (green), and glucagon (red). Scale bars: 50 µm. (b) Quantification of the total number of cells per islet section, with cell counts obtained through both manual counting and SAM-based analysis (n = 37 islet sections). (c) Quantification of insulin-positive cells per islet section, comparing manual counting and SAM-based quantification (n = 37 islet sections). (d) Quantification of glucagon-positive cells per islet section, with both manual counting and SAM analysis methods (n = 37 islet sections). (e) Quantification of bihormonal cells (cells positive for both insulin and glucagon) per islet section, evaluated using manual counting and SAM-based quantification (n = 37 islet sections).
