Fig. 4
From: Cooperative role of MDSCs and Tregs in alum-induced alloimmune tolerance in corneal transplantation
Alum-induced MDSCs modulate T cell responses in corneal allograft recipients. (A) Representative flow cytometry plots showing the percentage of Treg cells in the draining lymph nodes of corneal allograft recipients across different treatment groups (Alum, MDSCs, and PBS). (B) Quantification of Foxp3⁺ Tregs as a percentage of CD4⁺ T cells in different treatment groups. Alum-treated mice exhibit a significantly higher proportion of Tregs compared to PBS-treated controls (*p < 0.05), while no significant difference (ns) is observed between MDSC-treated and PBS-treated groups. (C) Cytokine profiling of CD4⁺ T cells from draining lymph nodes. Alum treatment significantly reduces pro-inflammatory cytokines IL-2, IFN-γ, and IL-17 A, while increasing IL-10, an immunoregulatory cytokine. Statistical significance: ***p < 0.001, **p < 0.01, *p < 0.05.
