Fig. 3
Tested lactonases are active in a variety of conditions including various coating chemistries, temperatures and salinity, and Acrylic coating with lactonase is shown to reduce biofilm. SsoPox (orange bars; A) and GcL (blue bars; B) (1 mg/mL) were combined with various coatings, and their paraoxonase activity was measured in acrylic (n = 3), silicone (n = 2), polyurethane (n = 3), epoxy (n = 3), and latex (n = 3) (see “Coating formulations and activity measurements” in methods). (C) SsoPox (orange) and GcL (blue) at concentrations between 0.2-5 mg/mL in an acrylic coating were tested for their biofilm reduction capabilities of Pseudomonas aeruginosa PAO1 using crystal violet (OD585 nm) (see “Biofilm Inhibition assay” in methods). Control indicates experiment with no enzyme treatment. Statistical significance was determined via a one-way ANOVA with a turkey multiple comparisons test. “ns” indicates p ≥ 0.05, *indicates p < 0.05, **indicates p < 0.01, ***indicates p < 0.001.
