Table 1 Details of the antibody and reagents used in this study.
Name of antibodies | Model | Norm | Factory owners | Operating concentration | Host species |
|---|---|---|---|---|---|
Anti-S100A9 + Calprotectin(S100A8/A9 complex) antibody | ab22506 | 10 µg | Abcam | 1 µg/ml (1/1000) | Mouse |
Anti-PD-L1 antibody | ab213524 | 40 µl | Abcam | 1/250 | Rabbit |
CD38 Antibody Reagent (immunohistochemistry) | MAB-0755 | 0.2 ml/bottle | Maixin | 1/100–1/200 | Mouse |
CD163 Antibody Reagent (immunohistochemistry) | MAB-0869 | 0.2 ml/bottle | Maixin | 1/100–1/200 | Mouse |
PD-1 Antibody Reagent (immunohistochemistry) | MAB-0734 | 0.2 ml/bottle | Maixin | 1/100–1/200 | Mouse |
Myeloperoxidase antibody reagent (immunohistochemistry) | RAB-0379 | 0.2 ml/bottle | Maixin | 1/50–1/100 | Rabbit |
BRAF V600E | – | 5 ml | Roche | 12 µg/ml | Mouse |
Ki67 | ZM-0167 | 0.2Â ml/vial | Zhongshan Golden Bridge | 1/200 | Mouse |
CD3 | ZM-0417 | 3Â ml/vial | Zhongshan Golden Bridge | Working solution | Mouse |
CD20 | ZM-0039 | 3Â ml/bottle | Zhongshan Golden Bridge | Working solution | Mouse |
CD68 | ZM-0060 | 3Â ml/vial | Zhongshan Golden Bridge | Working solution | Mouse |
β-catenin | ZM-0442 | 3 ml/vial | Zhongshan Golden Bridge | Working solution | Mouse |