Fig. 3 | Scientific Reports

Fig. 3

From: In-cell NMR reveals the first direct observation of endogenous interaction between HIV Tat protein and Tat RNA aptamer in human cells

Fig. 3

In vitro titration of HIV-1 Tat protein into Tat RNA aptamer monitored by 1D Imino proton NMR. 1H NMR spectra were recorded during the titration of purified HIV-1 Tat protein into the Tat RNA aptamer at 20 °C. Each spectrum corresponds to an incremental concentration of Tat protein, revealing progressive chemical shift perturbations indicative of complex formation. The boxed region highlights specific imino proton signals that undergo notable changes upon binding, confirming direct interaction between the aptamer and Tat protein.

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