Fig. 5 | Scientific Reports

Fig. 5

From: In-cell NMR reveals the first direct observation of endogenous interaction between HIV Tat protein and Tat RNA aptamer in human cells

Fig. 5

In-cell NMR of Tat RNA aptamer in complex with HIV-1 Tat protein. (A) Two-dimensional ¹H–¹⁵N HSQC spectrum acquired from HeLa cells expressing HIV-1 Tat protein and transfected with uniformly ¹⁵N-labeled Tat RNA aptamer. The appearance of imino cross-peaks confirms the intracellular folding and stabilization of the aptamer in the cellular environment. (B) Two-dimensional ¹H–¹H NOESY spectrum recorded under the same conditions reveals distinct cross-peaks corresponding to short-range spatial contacts within the RNA aptamer and at the RNA–protein interface. The presence of intra- and intermolecular NOEs confirms stable complex formation in the intracellular milieu. All spectra were acquired at 20 °C .

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