Fig. 6

Confocal microscopy of Tat RNA aptamer and HIV-1 Tat protein in HeLa cells. HeLa cells expressing TAMRA-tagged Tat protein were electroporated with FAM-labeled RNA aptamer. Confocal images show (A) brightfield, (B) Tat protein (TAMRA), (C) RNA aptamer (FAM), (D) merged Tat and aptamer signals, and (E) overlay with Hoechst-stained nucleus. Scale bar = 10 μm.