Fig. 3
From: Comparative and pharmacological investigation of bEVs from eight Lactobacillales strains
Enhanced collagen production by bEVs. A. Collagen staining in bEV-treated NIH3T3 cells. Collagen is stained with Sirius Red. Scale bar = 200 μm. B. Collagen quantification in NIH3T3 cells. C. Dose-dependent collagen production in NIH3T3 cells by selected bEVs. The extracted and quantified Sirius Red is shown on the left, and the amount of collagen is measured by ELISA on the right. D. Collagen synthesis and key molecules involved in collagen homeostasis in NIH3T3 cells. TGF-β is used as a positive control. E: Collagen staining in bEV-treated LX-2 cells. Scale bar = 200 μm. F: Collagen quantification in LX-2 cells. G. Dose-dependent collagen production in LX-2 cells by selected bEVs. H. Collagen synthesis and homeostasis axis activity in LX-2 cells. H, M, and L indicate high, middle, and low concentrations of bEVs, respectively, corresponding to three times, one time, and one-third of MTD. The data are presented as the means ± SDs; n = 3 ~ 8; *p < 0.05. **p < 0.01, ***p < 0.001 vs. vehicle control (Ctrl), one-way ANOVA followed by Dunnett’s test.
