Fig. 4
The expression of FEN1 and the regulation of FEN1 on PCNA in SCNECC. A Immunohistochemical detection and expression analysis of FEN1 in SCNECC. B Expression levels were scored and analyzed, with statistical significance indicated by asterisks compared to adjacent non-cancerous tissues. C The interaction network of FEN1 was obtained from the STRING database. D TC-YIK cells were transfected with either negative control (NC) siRNA or FEN1-specific siRNA for 24 h. Relative mRNA expression levels of FEN1 and PCNA were detected by qRT-PCR, with GAPDH serving as the internal reference gene. n = 3. E Following transfection of TC-YIK cells with NC or FEN1 siRNA for 48 h, protein expression levels of FEN1 and PCNA were examined by WB analysis. Grayscale analysis and statistical evaluation of the WB results were performed using ImageJ software. Two-independent-samples t-tests was employed to analyze the experimental data.
